This toolkit seeks to offer advice to researchers to enhance gender equality in research. The objective is to make researchers more sensitive to the gender dimension in science by helping them to remove sexist prejudices in research projects and understand the importance of creating mixed research teamsand bear in mind the gender approach in the outcomes of their research. The recommendations are applied to numerous research fields: health, food, agriculture, biotechnology, nanoscience, new technologies, energy, the environment, transport, socioeconomic sciences and human sciences.